Abstract The search for treatments that accelerate the healing of lesions is of constant interest. Matricaria recutita (chamomile) is a plant with antimicrobial, anti-inflammatory, and healing properties, and antimicrobial Photodynamic Therapy (aPDT) eradicates microorganisms, which favors tissue repair. Objective This study aimed to evaluate the effect of the topical use of chamomile with or without aPDT on tissue repair in rats’ tongues. Methodology A total of 75 male Wistar rats underwent standardized ulceration on the dorsum of the tongue using a punch of 5 mm diameter and were randomly allocated into the following groups: control (G1), chamomile fluid extract (G2), chamomile infusion (G3), aPDT (G4), and chamomile infusion + aPDT (G5). On the 3rd, 7th, and 14th days postoperatively, euthanasia was performed, and the ulcers were measured using calipers. The presence of edema, inflammatory infiltrate, cellularity, re-epithelialization, and characterization of total collagen were evaluated using sections stained with Hematoxylin and Eosin and Red Sirius. Histomorphometry analyses of the percentage of total collagen, the distance from the basal layer to the epithelial surface, and the thickness of the stratum corneum were performed. Descriptive (absolute/relative frequencies and modes) and exploratory analyses were performed. The associations between the groups and the presence of ulcers were analyzed with Fisher’s exact test. All analyses were performed using the R program and statistical significance was set at p=0.05. Results The G2 positively modulated the exudative and proliferative phases of repair, both clinically (p<0.0001) and histologically, whether in descriptive or inferential analyses (p<0.05). The G3 showed a significant difference in clinical parameters compared with G1 (p<0.0001). The G4 and G5 did not positively modulate tissue repair. Conclusion The chamomile fluid extract showed better outcomes for tissue repair in the rat tongue.

Resumo A síndrome mão-pé (SMP) é um efeito adverso comum da terapia anticâncer. Sabe-se que causa sintomas dermatológicos, incluindo eritema acral e disestesia das palmas das mãos e solas dos pés, inchaço, dor, coceira e descamação. Alguns medicamentos como a capecitabina são conhecidos por desencadear essa condição. No entanto, a pigmentação da mucosa oral é um efeito adverso raro. Este trabalho tem como objetivo relatar um caso de hiperpigmentação da mucosa oral causada pela terapia com capecitabina antes do diagnóstico clínico de SMP. Mulher de 58 anos, com diagnóstico de carcinoma invasivo de ducto mamário, apresentou metástase no sistema nervoso central, fígado, pele e pulmão, em uso de capecitabina todos os dias por 14 ciclos. O exame oral revelou máculas negras multifocais no palato duro, mucosa bucal bilateral, mucosa gengival e dorso de língua. A hipótese clínica foi de hiperpigmentação da mucosa oral pelo uso de capecitabina e apenas o acompanhamento periódico foi necessário. A hiperpigmentação da mucosa oral pela capecitabina é uma consequência rara da terapia neoplásica e sua associação com SMP não é clara e pouco relatada. O relato desses eventos é importante para alertar as equipes de saúde oncológica sobre a tolerância individual à terapia com capecitabina.

Abstract Hand-foot syndrome (HFS) is a common adverse effect of anticancer therapy. It is known to cause dermatological symptoms including acral erythema and dysesthesia of the palms and soles of the feet, swelling, pain, itching, and scaling. Some drugs, like capecitabine, are known to trigger this condition. However, pigmentation of the oral mucosa is a rare adverse effect. This study aims to report a case of oral mucosa hyperpigmentation caused by capecitabine therapy before the clinical diagnosis of HFS. A 58-year-old female, diagnosed with invasive breast duct carcinoma, had the central nervous system, liver, skin, and lung metastasis, using capecitabine every day for 14 cycles. Oral examination revealed multifocal black macules on the hard palate, bilateral buccal mucosa, gingival mucosa, and dorsum of the tongue. The clinical hypothesis was oral mucosa hyperpigmentation by capecitabine use and only periodic follow-up was necessary. Hyperpigmentation of oral mucosa by capecitabine is a rare consequence of neoplastic therapy and your association with HFS is unclear, and poorly reported. The report of these events is important to alert oncology health teams about the individual tolerance to capecitabine therapy.

RESUMO Objetivo: Corpos de Civatte são corpos coloidais de queratinócitos apoptóticos localizados na junção dermoepidérmica e que são frequentes em várias dermatoses, inclusive as lesões de líquen plano. O objetivo do presente trabalho foi determinar a presença de corpos de Civatte em uma amostra de biópsias incisionais obtidas a partir de pacientes com diagnóstico de líquen plano oral (LPO). Métodos: Tratou-se de um estudo transversal retrospectivo, realizado com 34 casos cujas lâminas foram coradas pela hematoxilina-eosina, pertencentes ao arquivo de um Laboratório de Patologia Oral de Instituição de Ensino Superior. A amostra obtida foi classificada em lesões brancas e vermelhas a partir dos dados clínicos disponíveis. As análises histológicas foram realizadas em microscópio de luz, nas objetivas de 10x e 40x, com identificação dos corpos de Civatte como presentes ou ausentes. Resultados: Os corpos colóides estavam presentes em 73,5% da amostra total estudada e em 74,2% das lesões brancas. Conclusão: Os corpos de Civatte foram frequentes em todas as amostras analisadas e não devem ser negligenciados como critério de diagnóstico histopatológico em lesões de LPO.

ABSTRACT Objective: Civatte bodies are colloid bodies of apoptotic keratinocytes located at the dermoepidermal junction and are common in several dermatoses, including lichen planus lesions. The present study aimed to determine the presence of Civatte bodies in a sample of incisional biopsies obtained from patients diagnosed with oral lichen planus. Methods: A retrospective cross-sectional study, carried out with 34 slides stained with hematoxylin-eosin from the archive of an Oral Pathology Laboratory of a Higher Education Institution. The sample obtained was classified into white and red lesions from the available clinical data. Histological analyses were performed under a light microscope, with 10x and 40x objectives, identifying the presence or absence of Civatte bodies. Results: Colloid bodies were present in 73.5% the total sample studied and in 74.2% white lesions. Conclusion: The Civatte bodies were frequent in all samples analysed and should thus be considered a criterion for histological diagnosis in lesions of Oral Lichen Planus.

Abstract Objective: To determine the relationship between tobacco and alcohol consumption and staging of head and neck cancer in patients treated at a referral hospital for cancer treatment. Material and Methods: This is a cross-sectional study based on medical records of patients with head and neck cancer treated between 2008 and 2015. The following variables were considered: gender, age, marital status, educational level, place of residence, profession, affected site, clinical staging of neoplasms, histopathological diagnosis, tobacco and alcohol consumption. Results: Of the 154 medical records analyzed, there was a predominance of male patients (72.7%), single (50%), with low education (88.1%), rural workers (38.3%), residents in the state countryside (70.2%) and aged 20-94 years. T3 and T4 size tumors prevailed. A significant correlation was found between alcohol use and tumor size (p=0.03) and presence of nodules (p<0.001), as well as between alcohol use and smoking with tumor size (p=0.04) and presence of nodules (p=0.019). No correlations were observed between tobacco use and injury staging. Conclusion: There was strong significance between tumor size and presence of nodules, both with the use of alcohol alone and with tobacco, thus suggesting the existence of important carcinogenic action of this substance.

ABSTRACT This study analyzed the epidemiological profile and the level of knowledge about oral mucositis and laser therapy in a population who performed chemotherapy treatment in a public health service (CICAN-BA) for the years of 2014 and 2015. Fifty handbooks analyzed and questionnaires applied to verify the patient's perception. In addition, examination of the oral cavity has allowed us to identify injuries resulting from side effects of chemotherapy on the oral mucosa. Seventy two per cent of the patients were female and the most prevalent type of cancer was breast cancer (62.06%). About 50% of patients only concluded elementary School and 42% had family income corresponding to the minimum wage. T2 and T4 tumors were more prevalent (72.21%). The oral examination revealed a higher frequency of periodontal disease and carious lesions (51.72%). To assess knowledge, 78% could not conceptualize mucositis and 72% did not use the laser as adjuvant therapy. The data indicate that most users of CICAN-BA are individuals of low income and education level, justifying the lack of understanding about cancer and its therapeutic approaches.

RESUMEN Este estudio tuvo como objetivo analizar el perfil epidemiológico y el nivel de conocimiento sobre la mucositis oral y laserterapia de una población que llevó a cabo el tratamiento de quimioterapia en un servicio de salud pública (CICAN-BA) en los años 2014 y 2015. Se analizaron 50 registros médicos y se aplicaron cuestionarios semiestructurados para verificar la percepción de los pacientes. Además, el examen de la cavidad bucal nos ha permitido identificar las lesiones resultantes de los efectos adversos de la quimioterapia en la mucosa oral. Se observó que la mayoría de los pacientes era del sexo femenino (72%) y el tipo más frecuente de cáncer fue el de mama (62.06%). Un 50% de los pacientes poseían solo estudios primarios y el 42% tenía ingreso familiar correspondiente a un salario mínimo. En esta muestra, se encontró un mayor porcentaje de tumores graduados como T2 y T4 (72.21%). El examen bucal reveló una mayor frecuencia de las enfermedades periodontales y las lesiones cariosas (51.72%). Al evaluar los conocimientos, el 78% no supo conceptualizar la mucositis y el 72% desconocían el uso del láser como terapia adyuvante. Así, los datos indican que la mayoría de los usuarios del CICAN/BA tiene bajos ingresos familiares y nivel de educación, lo que puede justificar la falta de comprensión sobre el cáncer y sus enfoques terapéuticos.

RESUMO O presente estudo objetivou analisar o perfil epidemiológico e o nível de conhecimento acerca da mucosite oral e laserterapia de uma população que realizou tratamento quimioterápico em um serviço público de saúde (CICAN-BA) nos anos de 2014 e 2015. Foram analisados 50 prontuários e aplicados questionários semiestruturados a fim de verificar a percepção dos pacientes. Adicionalmente, o exame da cavidade bucal permitiu identificar lesões resultantes dos efeitos adversos da quimioterapia sobre a mucosa oral. Observou-se que a maioria dos pacientes era do sexo feminino (72%) e o tipo de câncer mais prevalente foi o de mama (62.06%). Cerca de 50% dos pacientes possuíam apenas o Ensino Fundamental e 42% tinha renda familiar correspondente a um salário mínimo. Nessa amostra, constataram-se maiores percentuais de tumores graduados como T2 e T4 (72.21%). O exame bucal evidenciou maior frequência de periodontopatias e lesões cariosas (51.72%). Ao avaliar o conhecimento, 78% não souberam conceituar a mucosite e 72% desconheciam o uso do laser como terapia adjuvante. Logo, os dados indicam que a maioria dos usuários do CICAN-BA é de baixa renda e baixo nível de escolaridade, fato este que pode justificar a falta de compreensão acerca do câncer e de suas abordagens terapêuticas.

A anemia de Fanconi (AF) é uma doença autossômica recessiva heterogênea que pode causar uma variedade de alterações congênitas e de desenvolvimento. As características mais importantes da AF são a falência progressiva de medula óssea e o desenvolvimento de condições malignas, como leucemia mielóide aguda e tumores sólidos. Este artigo relata o caso de um paciente de 12 anos com AF atendido no Serviço de Estomatologia e Prevenção do Câncer Bucomaxilofacial do Hospital São Lucas (Brasil), que havia sido submetido a transplante de medula óssea (TMO) aos 5 anos e apresentaava lesões características da doença do enxerto versus hospedeiro (DEVH) crônica. O paciente foi regularmente acompanhado no Serviço e, 11 anos após a realização do TMO, desenvolveu um carcinoma epidermóide de língua, considerado com sendo uma condição não-tratável. O pactiente faleceu por asfixia poucos meses depois aos 16 anos. As razões para a ocorrência dessa condição maligna ainda são incertas, podendo estar relacionadas com a instabilidade cromossômica característica da AF, com fatores relacionados ao TMO ou com a DEVH. O acompanhamento sistemático desses pacientes permite a realização de abordagens terapêuticas precoces e menos agressivas quando necessário.

Fanconi anemia (FA) is an autosomal recessive disorder that might cause a variety of congenital and developmental abnormalities. The most important features of FA are progressive bone marrow failure and development of malignancies, particularly acute myeloid leukemia and solid tumors. This paper reports the case of a 12-year-old patient with FA assisted at the Stomatology and Bucomaxillofacial Cancer Prevention Service of São Lucas Hospital, Brazil, who had been submitted to bone marrow transplantation (BMT) at the age of 5 and exhibited oral lesions characteristic of chronic graft versus host disease (GVHD). The patient was treated and followed-up for the oral lesions. Eleven years after the BMT, he developed squamous cell carcinoma of the tongue with an aggressive behavior, which was considered an untreatable condition. The patient died few months later from asphyxia at the age of 16. The reasons for development of these malignant conditions are unknown. However, chromosomal instability typically observed in FA cases, BMT factors and GVHD have been considered. Systematic follow-up of these patients allows early and less invasive therapeutic approaches.

A matriz extracelular no carcinoma epidermóide da cavidade oral sofre modificações qualitativas e quantitativas durante a sua progressão. Estas alterações parecem possibilitar os processos de invasão e metástase que caracterizam as neoplasias malignas e são mais evidentes na interface tumor-hospedeiro. No nosso estudo, 23 biópsias incisionais de carcinomas epidermóides de diversas localizações da cavidade oral foram classificadas segundo o sistema de graduação histológica preconizado por Anneroth et al.¹ (1987). Foram utilizadas as colorações pela H. E. e a do tipo picrosirius, específica para colágeno. A expressão imuno-histoquímica do colágeno tipo I foi analisada em lesões com diferentes escores histológicos de malignidade. Pôde-se concluir que o colágeno apresenta diferentes padrões morfológicos, histoquímicos e imuno-histoquímicos em lesões com altos e baixos escores de malignidade.

An analysis of the extracellular matrix at the invasive front of squamous cell carcinoma of the oral cavity may improve the understanding of tumour cell matrix interactions during malignancy growth. Alterations in collagen I expression may influence cellular invasion and metastasis. In this work, 23 cases of squamous cell carcinoma were submitted to the Anneroth's malignancy grading system¹. H. E. and sirius red staining were used. Immunohistochemical expression of collagen type I protein was observed in different malignancy scores. As a result, it was observed that the extracellular matrix in squamous cell carcinoma of the oral cavity shows different patterns of collagen I expression in low and high scores of malignancy.